FN1 expression is a marker of radioresistance in head and neck squamous cell carcinoma

The goal of this project was to analyze differential expression in head and neck cancer cells with various intrinsic radiosensitivity. The gene expression profiles of the cell lines were determined using the Human Genome U133 plus 2.0 Arrays (Affymetrix, Santa Clara, CA). The UTSCC cells were cultured in DMEM (Gibco) supplemented with 2mM glutamine, 1% non-essential amino acids, 100 IU/ml penicillin-G, 50ug/ml streptomycin, and 5% fetal bovine serum (all from Gibco, Paisley, UK). Total RNA was prepared from 3 x 10^6 cells using the RNeasy Mini Protocol (Qiagen GmbH, Hilden, Germany). The study included a total of 10 samples with 2 seperate experiments from 5 cell lines respectivley.

本项目旨在分析不同内在放射敏感性的头颈癌细胞中的基因差异表达。研究采用人类基因组U133 Plus 2.0基因芯片（Human Genome U133 plus 2.0 Arrays，Affymetrix公司，美国加利福尼亚州圣克拉拉）对各细胞系的基因表达谱进行测定。UTSCC细胞系采用添加了2mM谷氨酰胺、1%非必需氨基酸、100 IU/ml青霉素G、50μg/ml链霉素及5%胎牛血清的DMEM培养基（Gibco，英国佩斯利）进行培养，上述试剂均购自Gibco品牌。本研究使用RNeasy迷你实验流程（Qiagen GmbH，德国希尔登），从3×10^6个细胞中制备总RNA。本研究共纳入10份样本，对应5株细胞系各2组独立实验。