Ribosome profiling of astrovirus-infected cells

Ribosome profiling (RiboSeq) is a high-throughput sequencing technique for globally mapping the positions of translating ribosomes on the transcriptome. We infected Caco2 cells with human astrovirus 1 (HAstV1). Cells were harvested at 12 hpi and either flash frozen with no pre-treatment (NT), or pre-treated with lactimidomycin for 30 minutes followed by flash freezing (LTM). These samples where then used for ribosome profiling.

核糖体谱（Ribosome profiling，RiboSeq）是一种用于在转录组范围内全局定位正在进行翻译的核糖体位置的高通量测序技术。本研究使用人类星状病毒1型（human astrovirus 1，HAstV1）感染Caco2细胞，于感染后12小时（hours post infection，hpi）收集细胞，并将收获的细胞分为两组：一组不进行预处理即快速冷冻（NT），另一组先用拉米多霉素预处理30分钟后再进行快速冷冻（LTM）。上述样本随后被用于核糖体谱分析。