Comparative analysis of transcriptome remodeling in plaque associated and plaque distant microglia during amyloid-ß pathology progression. Comparative analysis of transcriptome remodeling in plaque associated and plaque distant microglia during amyloid-ß pathology progression

In this study, we combined cell-specific laser capture and RNA-seq analysis to investigate transcriptome changes in both amyloid-ß plaque-associated and plaque-distant microglia at different stages of the disease. Overall design: Comparative gene expression profiling analysis of RNA-seq data for microglia isolated from APP/PS1 AD mice at 4, 8 and 12 months of age.

本研究结合细胞特异性激光捕获技术与RNA测序（RNA-seq）分析，探究疾病不同阶段中，与淀粉样β斑块（amyloid-ß plaque）相邻及远离斑块的小胶质细胞（microglia）的转录组（transcriptome）变化。
实验设计概述：对4月龄、8月龄及12月龄APP/PS1阿尔茨海默病（Alzheimer's Disease，AD）小鼠分离得到的小胶质细胞的RNA-seq数据进行比较基因表达谱分析。