Intestinal flora date-Group_NCD_HFD_LFBE.zip

All high-quality sequencing reads were clustered using USEARCH (v7.0.1090) with 97% similarity, and operational taxonomic units (OTUs) were obtained with a certain threshold. Each OTU was selected as the representative sequence and subjected to RDP Classifier software (v2.2) for taxonomical assignment. The number of sequences per sample was corrected for differences in sequencing depth between samples by rarefication, that is, the same number of reads is randomly subsampled in each sample. Second, the absolute number of sequences of each OTU in each sample was converted to relative abundance to reduce the effect of differences in sequence reads. The representative sequences, together with the abundance data, were used for taxonbased analysis.

所有高质量测序读段均使用USEARCH（v7.0.1090）以97%相似性完成聚类，并通过特定阈值筛选得到操作分类单元（operational taxonomic units，OTU）。选取每个OTU作为代表序列，利用RDP分类器（RDP Classifier，v2.2）进行分类学注释。通过稀疏化（rarefication）校正不同样本间的测序深度差异，即对每个样本随机抽取等量的测序读段，统一各样本的序列数量。其次，将每个样本中各OTU的绝对序列数转换为相对丰度，以降低测序读段数差异带来的影响。将代表序列与丰度数据相结合，用于基于分类单元的分析。