Multifaceted rewiring of the hepatic lipidome during proliferation and carcinogenesis - PART 1 (Liver Regeneration)

The scope of this project is to study, using state-of-the-art systems biology approaches integrating the changes in metabolomics, lipidomics and transcriptomics, changes in hepatocytes' metabolism occurring in liver regeneration. We focused on altered metabolic pathways including lipogenesis, fatty acid desaturation, and generation of phosphatidylcholine (PC) occurring in the liver following partial hepatectomy (PH) or carbon-tetrachloride (CCl4) injection. Overall design: Male C57BL/6J mice were challenged with A) IP injection of 2ul/g body weight of olive oil (OO) or CCl4:olive oil [1:1 (v/v)] mix; Mice were culled 3 days after IP injection. B) Partial Hepatectomy; Mice were culled 3 days after surgery.

本项目旨在采用整合了代谢组学（metabolomics）、脂质组学（lipidomics）与转录组学（transcriptomics）变化的前沿系统生物学方法，探究肝再生过程中肝细胞的代谢改变。本研究重点关注部分肝切除术（partial hepatectomy, PH）或四氯化碳（carbon-tetrachloride, CCl₄）注射后，肝脏内发生的异常代谢通路，涵盖脂肪生成、脂肪酸去饱和及磷脂酰胆碱（phosphatidylcholine, PC）的合成过程。整体实验设计如下：
A) 对雄性C57BL/6J小鼠予以腹腔注射（intraperitoneal injection, IP）处理：注射剂量为每克体重2微升橄榄油（olive oil, OO），或四氯化碳与橄榄油按1:1体积比混合的溶液；于注射后3天处死小鼠。
B) 对雄性C57BL/6J小鼠实施部分肝切除术；于术后3天处死小鼠。