Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and smyd4 mutant zebrafish cardiac transcriptomes. Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and smyd4 mutant zebrafish cardiac transcriptomes

Heart tissue mRNA profiles of 50 wild-type (WT) and 50 homozygous smyd4 mutant zebrafish embryos at 72-hpf created by CRISPR/Cas9 were generated by deep sequencing. Overall design: Heart mRNA profiles of 72-hpf wild type (WT) and smdy4 mutant zebrafish were generated by deep sequencing using illumina

本数据集通过深度测序技术，生成了50例野生型（WT）与50例经CRISPR/Cas9编辑获得的纯合smyd4突变型斑马鱼胚胎在受精后72小时（72-hpf）的心脏组织mRNA表达谱。
实验整体设计：采用Illumina测序平台，对受精后72小时的野生型斑马鱼与smyd4突变型斑马鱼的心脏组织mRNA表达谱进行深度测序，以获取对应的表达谱数据。