Evaluation of the anti-osteoporotic effect of Ginkgo biloba L. in Wistar rats with glucocorticoid-induced-osteoporosis by bone densitometry using dual-energy x-ray absorptiometry (DEXA) and mechanical testing

ABSTRACT Evaluate the effect of the extract of Ginkgo biloba in the bone alkaline phosphatase, bone mineral density, in the mechanical properties of the tibia in rats with glucocorticoid-induced-osteoporosis. After osteoporosis induction, the rats were divided into five groups: Osteoporosis; EGb1 (28 mg/Kg); EGb2 (56 mg/Kg); alendronate (0.2 mg/animal) and control. The animals were treated during 20 and 30 days. The control group was compared with the osteoporosis’s (Student’s t-test), while the other were analyzed by ANOVA test followed by Tukey/Dunnett’T3 (p<0.05). In the osteoporosis group the bone alkaline phosphatase, bone mineral density, the bone stiffness, the maximum load and the resilience were reduced. The bone alkaline phosphatase values increased in the EGb1 and EGb2 groups (30 days). In addition, in the EGb2 and alendronate groups (20 and 30 days) the bone mineral density increased. The extract of Ginkgo biloba restored bone alkaline phosphatase and bone mineral density using dual-energy x-ray absorptiometry.

摘要 本研究旨在评估银杏叶提取物对糖皮质激素诱导骨质疏松大鼠的骨碱性磷酸酶（bone alkaline phosphatase）、骨密度及胫骨机械力学性能的影响。大鼠造模成功后，被分为五组：骨质疏松模型组、EGb1组（28mg/kg）、EGb2组（56mg/kg）、阿仑膦酸钠组（0.2mg/只）及空白对照组。各组分别给药处理20天与30天。以Student’s t检验（Student’s t-test）比较空白对照组与骨质疏松模型组，其余组别采用单因素方差分析（ANOVA），后续辅以Tukey/Dunnett’T3检验，以p<0.05为差异具有统计学意义。结果显示，骨质疏松模型组大鼠的骨碱性磷酸酶活性、骨密度、胫骨刚度、最大载荷及弹性恢复能力均显著降低。EGb1与EGb2组大鼠在给药30天后，骨碱性磷酸酶活性显著升高。此外，EGb2组与阿仑膦酸钠组大鼠在给药20天及30天后，骨密度均明显提升。本研究采用双能X线骨密度吸收法（dual-energy x-ray absorptiometry）检测证实，银杏叶提取物可有效改善糖皮质激素性骨质疏松大鼠的骨碱性磷酸酶活性与骨密度。