Table_2_Whole-genome sequencing of marine water-derived Curvularia verruculosa KHW-7: a pioneering study.xlsx

Marine microorganisms are renowned for being a rich source of new secondary metabolites that are significant to humans. The fungi strain KHW-7 was isolated from the seawater collected from the Gulf of Khambhat, India, and identified as Curvularia verruculosa KHW-7. On a next-generation sequencing platform, C. verruculosa KHW-7’s whole-genome sequencing (WGS) and gene annotation were carried out using several bioinformatic methods. The 31.59 MB genome size, 52.3% GC, and 158 bp mean read length were discovered using WGS. This genome also contained 9,745 protein-coding genes, including 852 secreted proteins and 2048 transmembrane proteins. The antiSMASH algorithm used to analyze genomes found 25 secondary metabolite biosynthetic gene clusters (BGCs) that are abundant in terpene, non-ribosomal peptide synthetase (NRPS), and polyketides type 1 (T1PKS). To our knowledge, this is the first whole-genome sequence report of C. verruculosa. The WGS analysis of C. verruculosa KHW-7 indicated that this marine-derived fungus could be an efficient generator of bioactive secondary metabolites and an important industrial enzyme, both of which demand further investigation and development.

海洋微生物素来是对人类具有重要应用价值的新型次级代谢产物的宝贵资源。本研究从印度坎贝湾采集的海水样本中分离得到真菌菌株KHW-7，经鉴定为疣孢弯孢霉（Curvularia verruculosa）KHW-7。采用下一代测序平台，通过多种生物信息学方法完成了该菌株的全基因组测序（whole-genome sequencing, WGS）与基因注释工作。经WGS分析，该菌株基因组大小为31.59 MB，GC含量为52.3%，平均读长为158 bp；其基因组共编码9745个蛋白编码基因，其中包含852个分泌蛋白编码基因与2048个跨膜蛋白编码基因。利用antiSMASH算法对基因组进行分析，共识别出25个次级代谢产物生物合成基因簇（secondary metabolite biosynthetic gene clusters, BGCs），这些基因簇富集于萜类、非核糖体肽合成酶（non-ribosomal peptide synthetase, NRPS）以及Ⅰ型聚酮合酶（polyketides type 1, T1PKS）相关通路。据我们所知，本研究为首次发布疣孢弯孢霉的全基因组序列报告。对疣孢弯孢霉KHW-7的全基因组测序分析表明，这株海洋来源真菌可作为生物活性次级代谢产物与重要工业酶的高效生产宿主，二者均有待进一步研究与开发利用。