Gene Expression Data of Adipose Stem Cells from Normal-Weight Polycystic Ovary Syndrome Women vs. Controls

In vitro studies of subcutaneous (SC) abdominal adipose stem cells (ASC) from women with polycystic ovary syndrome (PCOS) show altered ASC commitment to preadipocytes and differentiation to mature adipocytes related to hyperandrogenism. The goal of the study is to use microarrays to examine whether SC abdominal ASC gene expression are altered in normal-weight PCOS women and correlated with hyperandrogenemia and/or insulin resistance, which are prevalent clinical pathologies of PCOS. ASCs were isolated from SC adipose tissue following SC abdominal biopsy in 4 PCOS women and 4 age and BMI matched controls for gene expression comparison. First generation of stem cells were cultured until cells reached confluency and isolated for RNA extraction and hybridization on Affymetrix microarrays. Grant#: P50 HD071836 Title: Androgen excess as a mechanism for adipogenic dysfunction in PCOS women Period: 2018-2022

针对多囊卵巢综合征（polycystic ovary syndrome, PCOS）女性的皮下（subcutaneous, SC）腹部脂肪干细胞（adipose stem cells, ASC）开展的体外研究显示，其向前脂肪细胞的定向分化以及向成熟脂肪细胞的终末分化均出现异常，且该异常与高雄激素状态（hyperandrogenism）相关。本研究旨在利用基因微阵列（microarrays）技术，探究体重正常的PCOS女性的皮下腹部ASC基因表达是否存在异常，以及该异常是否与PCOS的常见临床病理特征——高雄激素血症（hyperandrogenemia）和/或胰岛素抵抗相关。研究人员从4名PCOS女性及4名年龄、体质量指数（body mass index, BMI）匹配的对照受试者中，经皮下腹部活检获取皮下脂肪组织并分离得到ASC，用于基因表达水平的对比分析。将第一代干细胞培养至细胞汇合状态后，收集细胞进行RNA提取，并在Affymetrix基因微阵列上完成杂交实验。资助项目编号：P50 HD071836
项目标题：高雄激素状态作为多囊卵巢综合征女性脂肪生成功能异常的机制
资助周期：2018-2022