shRNA-mediated silencing of candidate pluripotency regulators in mouse epibladst stem cells. shRNA-mediated silencing of candidate pluripotency regulators in mouse epibladst stem cells

EpiSC-5 mouse epiblast stem cells were transduced with lentiviral vectors carrying shRNA for MR candidate genes or non-targeting controls. Expression profile was obtained 72h after transduccion. Overall design: EpiSC-5 mouse epiblast stem cells were transduced with lentiviral vectors carrying shRNA for candidate pluripotency regulators on on-targeting controls. mRNA was isolated 72h after transduccion and profiled using PLATE-Seq (PMID 28740083).

将EpiSC-5小鼠上胚层干细胞以携带靶向MR候选基因的短发夹RNA(shRNA)或非靶向对照的慢病毒载体(lentiviral vectors)进行转导，于转导72小时后获取细胞表达谱。总体实验设计：将EpiSC-5小鼠上胚层干细胞以携带靶向候选多能性调控因子的短发夹RNA(shRNA)或非靶向对照的慢病毒载体进行转导，于转导72小时后分离信使RNA(mRNA)，采用PLATE-Seq技术（PubMed ID：28740083）完成表达谱分析。