Figure 1 source data

This dataset is part of the 'Source data and representative raw data for "Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis"' collection. For a link to the related paper, study-level information and guide to the data, as well as viewing all other related research data, please visit the collection. This data is associated with Figure 1 of the paper in Nature Microbiology. It contains videos of vertically trapped Bacillus subtilis cells expressing fluorescent proteins FtsZ and PBP2B, showing single-molecule tracking of the latter around the division ring. It also contains data from further down the processing and analysis pipeline from the same videos. 220303_slide2_5_MMStack_Default_ring34.ome corresponds to Fig. 1b, left panels. 220309_6_MMStack_Default_ring5.ome corresponds to Fig. 1b, right panels. 220316_5_MMStack_Default_561_denoise.ome_ring15 corresponds to Fig. 1c, top kymograph. 220316_5_MMStack_Default_561_denoise.ome_ring14 corresponds to Fig. 1c, bottom kymograph. 220412_slide2_6_MMStack_Default_561_denoise.ome_ring11 corresponds to Fig. 1d, top kymograph. 220414_4_MMStack_Default_561_denoise.ome_ring3 corresponds to Fig. 1d, bottom kymograph. 220809_sh147_phmm_30c_vercini_fosfomycin_slide2_006_561_denoise_ring1 corresponds to Fig. 1e, top kymograph. 220809_sh147_phmm_30c_vercini_fosfomycin_slide2_001_561_denoise_ring8 corresponds to Fig. 1e, bottom kymograph. 230613_sh147_speeds_phmm30_gauss_2nmsbin and 230605_sh147_speeds_phmm30_logscale correspond to Fig. 1f. 230614_sh147_phmm30_penG_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_penG_logscale correspond to Fig. 1g. 230614_sh147_phmm30_fosfomycin_2foldedgauss_2nmsbin and 230605_sh147_speeds_phmm30_fos_logscale correspond to Fig. 1h. Whitley_2024_Fig1_SourceData contains all the data used to produce histograms in Fig. 1f, 1g, and 1h.

本数据集隶属于《肽聚糖合成驱动枯草芽孢杆菌（Bacillus subtilis）分裂过程中分隔细胞壁合成酶的单群体行为》一文配套的源数据与代表性原始数据集合集。如需获取相关论文链接、研究级数据说明与数据使用指南，或浏览所有其他关联研究数据集，请访问该合集页面。本数据集对应发表于《自然·微生物学》（Nature Microbiology）的论文中的图1，其中包含垂直捕获的表达荧光蛋白FtsZ与PBP2B的枯草芽孢杆菌细胞视频，展示了后者在分裂环周围的单分子追踪过程；同时还包含来自上述相同视频的后续处理与分析流程所得到的数据。各数据文件与图版的对应关系如下：220303_slide2_5_MMStack_Default_ring34.ome 对应图1b左侧面板；220309_6_MMStack_Default_ring5.ome 对应图1b右侧面板；220316_5_MMStack_Default_561_denoise.ome_ring15 对应图1c顶部线扫描图像（kymograph）；220316_5_MMStack_Default_561_denoise.ome_ring14 对应图1c底部线扫描图像；220412_slide2_6_MMStack_Default_561_denoise.ome_ring11 对应图1d顶部线扫描图像；220414_4_MMStack_Default_561_denoise.ome_ring3 对应图1d底部线扫描图像；220809_sh147_phmm_30c_vercini_fosfomycin_slide2_006_561_denoise_ring1 对应图1e顶部线扫描图像；220809_sh147_phmm_30c_vercini_fosfomycin_slide2_001_561_denoise_ring8 对应图1e底部线扫描图像；230613_sh147_speeds_phmm30_gauss_2nmsbin 与 230605_sh147_speeds_phmm30_logscale 对应图1f；230614_sh147_phmm30_penG_2foldedgauss_2nmsbin 与 230605_sh147_speeds_phmm30_penG_logscale 对应图1g；230614_sh147_phmm30_fosfomycin_2foldedgauss_2nmsbin 与 230605_sh147_speeds_phmm30_fos_logscale 对应图1h；Whitley_2024_Fig1_SourceData 包含用于生成图1f、1g及1h中直方图的全部数据。