Genome-wide analysis of SRC-1/NCOA1 and Era/ESR1 binding in chromatin obtained from the ERa stably transfected U2OS osteosarcoma cell line (U2OS-ERa) with ChIP-SEQ.

To better understand the role of SRC-1 in estrogen signaling in bone, a genome-wide analysis of ERa and SRC-1 binding sites in the absense and presence of E2 was conducted. Overall design: Cells were grown in CSS media for 3 days, and treated with vehicle (EtOH) or estrogen (10^-8M) for 45 minutes and immediately harvested for chomatin immunoprecipitation (ChIP)

为深入解析类固醇受体共激活因子1（SRC-1）在骨骼雌激素信号通路中的功能，本研究针对雌二醇（E2）存在与缺失两种条件下，雌激素受体α（ERα）与SRC-1的全基因组结合位点开展了系统分析。
实验设计方案：将细胞于活性炭剥离血清（CSS）培养基中培养3天，随后分别以溶剂对照（乙醇，EtOH）或10^-8M浓度的雌激素处理45分钟，即刻收集样本以进行染色质免疫沉淀（ChIP）实验。