Characterization of a spontaneous model of inflammation-derived liver cancer [RNA-seq]. Mus musculus

Induction of a protective response to oxidative stress, both from a transcriptional and from a genetic viewpoint, confers a selective advantage for cells exposed to an inflammatory environment, paving the way to neoplastic transformation Overall design: Sequencing of RNA extracted from tissues of Mdr2-WT (FVB.129P2) livers, Mdr2-KO (FVB.129P2-Abcb4tm1Bor/J) inflamed livers or hepatocarcinoma nodules from Mdr2-KO livers. All samples have been treated with clodronate liposomes to prevent sample contamination by liver macrophages

从转录与遗传学层面诱导氧化应激保护性反应，可为暴露于炎症环境的细胞赋予选择优势，进而为肿瘤转化铺平道路。实验设计：对Mdr2野生型（FVB.129P2）小鼠肝脏组织、Mdr2基因敲除型（FVB.129P2-Abcb4tm1Bor/J）小鼠炎症肝脏组织，以及Mdr2基因敲除小鼠肝脏中的肝癌结节所提取的RNA进行测序。所有样本均经氯膦酸盐脂质体处理，以避免肝脏巨噬细胞对样本造成污染。