In Vitro Antimicrobial Effect of Bioadhesive Oral Membrane with Chlorhexidine Gel

Abstract This study aimed to evaluate in vitro the antimicrobial effect of a bioadhesive chitosan-based oral membrane with chlorhexidine for local treatment of infections in the oral tissues. Five oral membranes of different compositions were tested: 5% chitosan (G1); 5% chitosan ± 0.2% chlorhexidine (G2), 5% chitosan ± 0.6% chlorhexidine (G3), 5% chitosan ± 1.0% chlorhexidine (G4), and 5% chitosan ± 2.0% chlorhexidine (G5). Also, five gel types were tested according to the following compositions: 5% chitosan gel (G6), 0.2% chlorhexidine gel (G7), 2.0% chlorhexidine gel (G8), 5% chitosan gel ± 0.2% chlorhexidine gel (G9), and 5% chitosan gel ± 2.0% chlorhexidine gel (G10). The antimicrobial action of the samples was tested against Candida albicans and Streptococcus mutans through antibiogram by measuring the inhibition halos. Data were statistically analyzed by Kruskal-Wallis and one-way ANOVA followed by Tukey test (p

摘要 本研究旨在体外评价负载氯己定（chlorhexidine）的生物粘附性壳聚糖基口腔膜剂的抗菌效果，用于口腔组织感染的局部治疗。本研究共测试了五种不同配方的口腔膜剂：5%壳聚糖组（G1）、5%壳聚糖±0.2%氯己定组（G2）、5%壳聚糖±0.6%氯己定组（G3）、5%壳聚糖±1.0%氯己定组（G4）以及5%壳聚糖±2.0%氯己定组（G5）。此外，本研究还按照以下配方测试了五种凝胶制剂：5%壳聚糖凝胶组（G6）、0.2%氯己定凝胶组（G7）、2.0%氯己定凝胶组（G8）、5%壳聚糖凝胶±0.2%氯己定组（G9）以及5%壳聚糖凝胶±2.0%氯己定组（G10）。本研究通过药敏试验（antibiogram）测量抑菌圈（inhibition halos）的方法，评价了受试样品对白色念珠菌（Candida albicans）和变形链球菌（Streptococcus mutans）的抗菌活性。采用克鲁斯卡尔-沃利斯检验（Kruskal-Wallis）、单因素方差分析（one-way ANOVA）结合Tukey检验对数据进行统计学分析（p值未完整给出）。