Plate 4

This item contains a zip file with 6,000 images from plate 4 and a metadata CSV file for this specific plate. <br> There are 4 channels/stains:<br> - <strong>DAPI</strong> (DNA/Nuclei)<br> - <strong>GFP</strong> (ER)<br> - <strong>CY5</strong> (Mitochondria)<br> - <strong>RFP</strong> (Actin)<br> <br> There are NF1 Null and WT cells on this plate, with only the WT cells either not treated or treated with one of three siRNA constructs to decrease neurofibromin production using a dose curve.<br> <br> For more information on the data, please go to the GitHub repository for this project, nf1_cellpainting_data.

本数据集包含一个压缩包，内含来自第4号实验板的6000张图像及对应该实验板的元数据CSV文件。<br>本数据集包含4种染色通道：<br> - <strong>4',6-二脒基-2-苯基吲哚（DAPI）</strong>：用于标记DNA/细胞核<br> - <strong>绿色荧光蛋白（GFP）</strong>：用于标记内质网（ER）<br> - <strong>花青5（CY5）</strong>：用于标记线粒体<br> - <strong>红色荧光蛋白（RFP）</strong>：用于标记肌动蛋白<br><br>该实验板包含NF1基因敲除（NF1 Null）与野生型（Wild Type，WT）细胞，其中仅野生型细胞未接受任何处理，或通过三种小干扰RNA（siRNA）构建体进行剂量梯度处理，以降低神经纤维蛋白的表达量。<br><br>如需了解该数据集的更多详细信息，请访问本项目的GitHub仓库nf1_cellpainting_data。