Agro_AD1231

AD1231: pAD1361/A136; Carbenicillin 50; virB, virD4, virGN54D on multicopy plasmid, host lacks a Ti-plasmid. Constitutive virG mutant, does not require AS. But good to have AS in the media, anyway.

AD1231菌株：携带pAD1361/A136质粒；培养基添加50 μg/mL羧苄青霉素（Carbenicillin）；其毒力相关多拷贝质粒上携带有virB、virD4及virGN54D突变基因，宿主菌株不含Ti质粒（Ti-plasmid）。该菌株为组成型表达的virG突变体，无需乙酰丁香酮（Acetosyringone，AS）诱导即可激活相关通路，但即便如此，在培养基中添加AS仍可获得更佳实验效果。