Sequencing results of vectors contains containing phototropin CDSs for expression in insect cells and bacteria

Results of sequencing of vectors with phototropin CDS: You can view all sequencing results as assembled contigous sequence of mutants of phototropin1 or phototropin2 at Gene Snap Viewer (www.snapgene.com/snapgene-viewer). Phototropins sequences represented by: phot1 CDS, phot2 CDS phot1 CDS – 2991 nucleotides phot2 CDS – 2748 nucleotides phot2LOV1LOV2 - N-terminal PHOT2 fragment, containing LOV1 and LOV2 – 1161 nucleotides Mutants: Phot1 1_pFASTphot1D806N – mutation of 1 nucleotide at 2416 position, 2_pFASTphot1C234A – mutation of 2 nucleotides at 700 and 701 positions, 3_pFASTphot1C512A – mutation of 2 nucleotides at 1534 and 1535 positions, 4_pFASTphot1C234AC512A – mutation of 2 nucleotides at 700 and 701 as well as at 1534 and 1535 positions, Phot2 5_pFASTphot2D720A – mutation of 1 nucleotide at 2158 position, 6_pFASTphot2C170A – mutation of 2 nucleotides at 508 and 509 positions, 7_pFASTphot2C426A – mutation of 2 nucleotides at 1276 and 1277 positions, 8_pFASTphot2C170AC426A – mutations of 2 nucleotides at 508 and 509 positions as well as at 1276 and 1277, phot2LOV1LOV2 fragment mutants 9_pDONR221phot2LOV1LOV2V392T – mutation of 2 nucleotides at 799 and 800 positions, 10_pDONR221phot2LOV1LOV2V392L – mutation of 1 nucleotide at 799 position, Phot2 without stop codon (2745 nucleotides) 11_pDONR221phot2V392T – mutation of 2 nucleotides at 1174 and 1175 positions, 12_pDONR221phot2V392L – working on Phot2 with stop codon (2748 nucleotides) 13_pDONR221phot2V392Tstop – mutation of 2 nucleotides at 1174 and 1175 positions, 14_pDONR221phot2V392Lstop – mutation of 1 nucleotide at 1174 position. Phot2 574aa stop (1722 nucleotides) 15_pDONR221phot2 574aaastop – wild type, 16_pDONR221phot2 574aaastop V392T – mutation of 2 nucleotides at 1174 and 1175 positions, 17_pDONR221phot2 574aaastop V392L – mutation of 1 nucleotide at 1174 position.

向光蛋白（phototropin）编码序列（Coding Sequence，CDS）载体的测序结果如下：所有测序结果均可在基因快照查看器（Gene Snap Viewer，网址：www.snapgene.com/snapgene-viewer）中查看，其为向光蛋白1（phototropin1，简写为phot1）或向光蛋白2（phototropin2，简写为phot2）突变体的组装连续序列。
向光蛋白序列涵盖以下类型：phot1 CDS、phot2 CDS；其中phot1 CDS长度为2991个核苷酸，phot2 CDS长度为2748个核苷酸；phot2LOV1LOV2为PHOT2的N端片段，包含LOV1与LOV2结构域，长度为1161个核苷酸。
突变体详情如下：
#### Phot1突变体
1_pFASTphot1D806N：在第2416位发生1个核苷酸突变；
2_pFASTphot1C234A：在第700、701位发生2个核苷酸突变；
3_pFASTphot1C512A：在第1534、1535位发生2个核苷酸突变；
4_pFASTphot1C234AC512A：同时在第700、701位以及第1534、1535位发生核苷酸突变；
#### Phot2突变体
5_pFASTphot2D720A：在第2158位发生1个核苷酸突变；
6_pFASTphot2C170A：在第508、509位发生2个核苷酸突变；
7_pFASTphot2C426A：在第1276、1277位发生2个核苷酸突变；
8_pFASTphot2C170AC426A：同时在第508、509位以及第1276、1277位发生核苷酸突变；
#### phot2LOV1LOV2片段突变体
9_pDONR221phot2LOV1LOV2V392T：在第799、800位发生2个核苷酸突变；
10_pDONR221phot2LOV1LOV2V392L：在第799位发生1个核苷酸突变；
#### 无终止密码子的Phot2（长度2745个核苷酸）相关载体
11_pDONR221phot2V392T：在第1174、1175位发生2个核苷酸突变；
12_pDONR221phot2V392L：针对携带终止密码子的Phot2（长度2748个核苷酸）的研发工作正在进行中；
#### 574氨基酸截短型Phot2（含终止密码子，长度1722个核苷酸）相关载体
13_pDONR221phot2V392Tstop：在第1174、1175位发生2个核苷酸突变；
14_pDONR221phot2V392Lstop：在第1174位发生1个核苷酸突变；
15_pDONR221phot2 574aaastop：野生型载体；
16_pDONR221phot2 574aaastop V392T：在第1174、1175位发生2个核苷酸突变；
17_pDONR221phot2 574aaastop V392L：在第1174位发生1个核苷酸突变。