Transcriptional profiling of shoots and roots in japonica and indica rice seedlings under salt stress and recovery stage. Oryza sativa

Rice seedlings at 3-leaf stage were used for expression analysis in control and salt stressed (incloudling salt treatment for 3, 24hrs and recovery from salt stress for 24hrs) samples. Samples of shoots and roots from biological replicates of both genotypes were generated and the expression profiles were determined using Phalanx Rice OneArray＠ v1. Overall design: Control and treated biological replicates of salt-tolerant cultivar TNG67 (japonica) and salt-sensitive cultivar TCN1 (indica) were analyzed

本研究以三叶期水稻幼苗为实验材料，对对照组及盐胁迫处理组样本开展基因表达分析；其中盐胁迫处理组涵盖盐处理3小时、24小时，以及盐胁迫解除后恢复培养24小时三种处理条件。采集两个水稻基因型材料的生物学重复样本的地上部与根部组织，采用Phalanx Rice OneArray@v1芯片完成表达谱检测。实验整体设计如下：分别对盐耐受粳稻（japonica）品种TNG67与盐敏感籼稻（indica）品种TCN1的对照组及处理组生物学重复样本进行分析。