一种鸭瘟活疫苗及其制备方法

鸭瘟病毒强毒株经无特定病原体（Specific pathogen free, SPF）鸡胚成纤维细胞（chick embryo fibroblast, CEF）连续传代80代，克隆纯化得到的弱毒株，命名为鸭肠炎病毒（duck enteritis virus）DEVC86株，该毒株已于2013年04月送交中国科学院微生物研究所中国微生物菌种保藏管理委员会普通微生物中心保藏，保藏编号CGMCC No. 7460。
本发明涉及一种鸭瘟活疫苗及其制备方法。所提供的鸭肠炎病毒是人工致弱的鸭肠炎病毒CGMCC No.7460（DEVC86）株，能在CEF繁殖，病毒含量高，制造疫苗方便；雏鸭安全，对鸡不致死，具有良好的生物安全性；免疫原性好，能有效地保护各品种鸭抵抗鸭瘟感染；该鸭肠炎病毒CGMCC No.7460株的基因组145818-147618位缺失区域按常规生物学技术插入不同的水禽病毒的保护性抗原基因片段构建成相应的重组病毒。

A highly virulent duck plague virus strain was serially passaged 80 times in specific pathogen-free (SPF) chick embryo fibroblast (CEF) cells, then cloned and purified to obtain an attenuated strain designated as duck enteritis virus (DEV) strain DEVC86. This strain was deposited to the China General Microbiological Culture Collection Center (CGMCC) under the China Committee for Culture Collection of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences in April 2013, with the deposition number CGMCC No. 7460.

The present invention relates to a live duck plague vaccine and its preparation method. The provided duck enteritis virus is the artificially attenuated duck enteritis virus strain CGMCC No.7460 (DEVC86), which can replicate in CEF cells with high viral titer, facilitating vaccine production. It is safe for ducklings, non-lethal to chickens, and exhibits excellent biosafety. It has good immunogenicity and can effectively protect ducks of all breeds against duck plague infection. Recombinant viruses can be constructed by inserting protective antigen gene fragments of different waterfowl viruses into the 145818-147618 bp deletion region of the genome of DEV strain CGMCC No.7460 using conventional biological techniques.