Expression profile of TTHA0344-deficient Thermus thermophilus HB8 strain. Thermus thermophilus HB8

In order to find function of TTHA0344 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0344-deficient strain grown in a rich medium at 70°C for 680 min. Overall design: Three TTHA0344-deficient T. thermophilus HB8 strains were each pre-cultured at 70°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C. Cells were collected after 680 min, and then, the expression of each mRNA was analyzed.

为解析嗜热栖热菌HB8（Thermus thermophilus HB8）TTHA0344蛋白的功能，我们对在丰富培养基中于70℃培养680分钟的TTHA0344缺陷菌株的总信使核糖核酸（total mRNA）表达谱进行了分析。实验整体设计如下：将3株TTHA0344缺陷型嗜热栖热菌HB8菌株分别接种于3 mL TT培养基中，该培养基含0.8%蛋白胨（polypeptone）、0.4%酵母提取物（yeast extract）、0.2%氯化钠（NaCl）、0.4 mM氯化钙（CaCl₂）、0.4 mM氯化镁（MgCl₂），以氢氧化钠（NaOH）调节pH至7.2，随后于70℃预培养16小时。取2 mL菌液接种至1 L相同培养基中，于70℃继续培养；培养680分钟后收集菌体，对各mRNA的表达水平进行分析。