Eragrostis curvula cultivar:Victoria Genome sequencing and assembly. Eragrostis curvula cultivar:Victoria

Here we present the first genome of E. curvula genome. The cultivar selected was a sexual diploid (620 Mb haplotype) originated from in vitro culture of inflorescences of the apomictic cv. Tanganyika (2n=4x=40). The sequencing has been done through PACBIO and after the assembly one Dovetail Chicago and one Dovetail Hi-C library was added obtaining chromosomes size contigs covering 95% of the diploid genome. The high proportion of annotated genes would allow the identification of those related to the reproductive mode. This genome is the start point to obtain more complex tetraploid genomes, harbouring the region/s involved in apomixis.

本研究首次公布弯叶画眉草（E. curvula）的基因组序列。本次选用的试验材料为一株有性生殖二倍体（单倍型大小为620 Mb），其来源于无融合生殖品种Tanganyika（2n=4x=40）的花序体外培养体系。测序工作通过PACBIO平台完成，基因组组装阶段额外添加一套Dovetail Chicago文库与一套Dovetail Hi-C文库进行辅助组装，最终获得覆盖该二倍体基因组95%序列的染色体级重叠群。该基因组注释得到的高比例功能基因，可用于鉴定与生殖模式相关的关键基因。本基因组可为后续构建更为复杂的四倍体基因组奠定基础，其携带了与无融合生殖相关的基因区域。