miR-30e* induced gene expression alteration in glioma cells

This experiment is designed to evaluate gene expression alteration following miR-30e* transfection in glioma cells. We find a significant elevation of NF kappa B transcriptional targets. Total RNA were extracted from SNB-19 and U-87MG glioma cells transfected with miR-30e* mimic oligo or miRNA mimic negative control 36 hours after transfection, two biological replications for each treatment.

本实验旨在评估胶质瘤细胞中转染miR-30e*后发生的基因表达改变。本研究发现核因子κB（NF κB）的转录靶基因存在显著上调。实验于转染后36小时，从转染了miR-30e*模拟寡核苷酸或miRNA模拟物阴性对照的SNB-19与U-87MG胶质瘤细胞中提取总RNA，每组处理均设置2次生物学重复。