Metadata file from the manuscript 'Alterations in airway microbiota in patients with acute lung injury after burn and inhalation injury'

Metadata file for 16S rRNA gene sequencing done on airway samples from victims of burn and inhalation injury.Duplicate barcoded paired-end reads were sequenced on the Illumina MiSeq using the molecule tagging method described by Lundberg et. al. (Nature Methods, 2013, DOI: 10.1038/nmeth.2634) and the OTU table was generated using the MTToolbox pipeline (Yourstone, 2015, BMC Bioinformatics, DOI: 10.1168/1471-2105-15-284). The OTU table was imported into the program Explicet (www.explicet.org; Robertson et. al., Bioinformatics, 2013, DOI: 10.1093/bioinformatics/btt526), OTUs identified as the same species were condensed, and this table was exported and used for analysis in R.

本文件为针对烧伤与吸入损伤受害者气道样本开展16S rRNA基因测序的元数据文件。研究采用Lundberg等（《Nature Methods》, 2013, DOI: 10.1038/nmeth.2634）所述的分子标记方法，在Illumina MiSeq平台上对带条形码的重复双端测序读段进行测序；依托MTToolbox分析流程（Yourstone, 2015, 《BMC Bioinformatics》, DOI: 10.1168/1471-2105-15-284）生成操作分类单元（OTU, Operational Taxonomic Unit）表。将该OTU表导入Explicet软件（www.explicet.org; Robertson等, 《Bioinformatics》, 2013, DOI: 10.1093/bioinformatics/btt526），对注释为同一物种的OTU进行合并，随后导出该表格并用于R语言环境下的后续分析。