CIL:12957, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and GOLPH3 (a trans-Golgi associated protein that can also be associated with mitochondria; smaller gold particles) staining. Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.

本数据集源自对泌乳大鼠乳腺组织的免疫电子显微镜研究，旨在探究成熟脂滴与反式高尔基网络（trans-Golgi network, TGN）各组分间的潜在结构相互作用。实验通过TGN38（一种跨膜反式高尔基蛋白，对应较大金颗粒）与GOLPH3（一种可同时与线粒体结合的反式高尔基相关蛋白，对应较小金颗粒）染色实现靶标组分识别。需注意，泌乳乳腺细胞内的反式高尔基潴泡富含酪蛋白及其他乳蛋白，且因分泌液的渗透压作用发生显著扩张。