IMC Matched Primary and Metastatic Breast Cancer

This repository contains all raw MCD files and the fully annotated dataset file (backup_output.rds) that pertain to imaging mass cytometry analysis of primary and metastatic breast cancers in the manuscript by Grasset et al. "Mapping the breast tumor microenvironment: proximity analysis reveals spatial relationships between macrophage subtypes and metastasis-initiating cancer cells". Otherlegends.zip contains the antibody panel, maps of ROI assignments for each of the 5 TMAs used, and legends/metadata related to all of the individual ROIs. R scripts used for the analysis of this dataset are available on https://github.com/wjhlab/BreastMetIMC/本仓库包含所有原始MCD文件与经完整注释的数据集文件（backup_output.rds），这些数据均与Grasset等人发表于论文《Mapping the breast tumor microenvironment: proximity analysis reveals spatial relationships between macrophage subtypes and metastasis-initiating cancer cells》中的原发性与转移性乳腺癌成像质谱流式细胞术（imaging mass cytometry, IMC）分析相关。 Otherlegends.zip 压缩包内含抗体组合（antibody panel）、所用5张组织微阵列（tissue microarray, TMA）各自的感兴趣区域（region of interest, ROI）分配图谱，以及与所有单个ROI相关的图例与元数据。 本数据集分析所用的R脚本可从https://github.com/wjhlab/BreastMetIMC/ 获取。