Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool92_h1930004_A1C

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人脑的细胞结构，对于理解其多样功能与复杂的动物行为至关重要。本研究对人脑多个脑区采用单细胞核甲基化测序（Single nucleus methylation sequencing）技术，鉴定得到多组特征各异的细胞簇，并构建了层级分类学框架。这些细胞簇既包含已被公认的主要脑细胞类型，也涵盖了潜在的细胞亚型。研究团队利用该数据集鉴定了各细胞簇的表观基因组特征，并明确了每个细胞簇对应的特异性调控元件。整体实验设计：对脑区开展snmC-seq3测序，以解析成年人类大脑的表观基因组图谱。