Role of YbeY in Yersinia enterocolitica O:3. Yersinia enterocolitica subsp. palearctica Y11

In order to detect genes that are directly or indirectly affected by the inactivation of the ybeY gene the transcriptomes of the WT and ybeY bacteria grown at 22°C and 37°C were determined. The two temperatures were chosen since temperature is an important cue regulating the virulence genes. When comparing the WT and ybeY mutant strain transcriptomes of bacteria grown at 22°C we found changes in the expression of 350 genes in the ybeY bacteria, out of which 121 (34.6%) were up-regulated and 229 (65.4%) down-regulated. At 37°C 334 genes in the ybeY bacteria showed changes, out of which 48 (14.4%) were up-regulated and 286 (85.6%) down-regulated. Expression of 92 genes was changed at both temperatures, of these 92 genes, 12 were up- and 80 down-regulated. Overall design: Bacterial RNA profiles of wild type and ybeY- strains were generated using deep sequencing, in duplicates, under two growth temperatures (22°C and 37°C).

为了检测受ybeY基因失活直接或间接影响的基因，我们对在22°C和37°C下培养的野生型（Wild Type, WT）及ybeY缺陷型细菌的转录组（transcriptome）进行了测定。选择这两个温度是因为温度是调控毒力基因表达的重要信号。
比对22°C下培养的野生型与ybeY突变菌株的细菌转录组，我们发现ybeY缺陷型细菌中有350个基因的表达发生变化，其中121个（34.6%）表达上调，229个（65.4%）表达下调。
在37°C下，ybeY缺陷型细菌中有334个基因的表达发生变化，其中48个（14.4%）表达上调，286个（85.6%）表达下调。
有92个基因的表达在两个温度下均发生改变，其中12个表达上调，80个表达下调。
总体实验设计：采用深度测序（deep sequencing）技术，在两种生长温度（22°C与37°C）下，对野生型及ybeY缺失菌株的细菌RNA进行双生物学重复实验，以获取其转录组表达谱。