Transcriptomic analysis of 52 hpf mbd5 KO & KD embryos and 7 dpf mb5 KO & OE larves

To dissect the molecular function of mbd5 in zebrafish embryonic development, we carried out transcriptomic analyses at those two developmental stages. Overall design: Gene expression profiles of mbd5 KD & KO and siblings at 52 hpf, mbd5 KO & OE and siblings at 7 dpf were generated by deep sequencing with Illumina HiSeq X Ten platform

为解析斑马鱼胚胎发育中mbd5的分子功能，我们在两个发育阶段开展了转录组分析。整体实验设计：采用Illumina HiSeq X Ten平台进行深度测序，获取了以下两组样本的基因表达谱：一是受精后52小时（52 hpf, hours post fertilization）的mbd5敲降（Knockdown, KD）、敲除（Knockout, KO）样本及其同窝对照；二是受精后7天（7 dpf, days post fertilization）的mbd5敲除（KO）、过表达（Overexpression, OE）样本及其同窝对照。