DNA methylation profiling of ductal carcinoma in situ (DCIS). Homo sapiens

Methylated genomic fragments from tumor DNA and matching normal control were enriched with the methylated-CpG island recovery assay (MIRA); amplified, labeled and hybridized on Agilent CpG island tiling oligo arrays. MIRA enriched samples from tumor were labeled with Cy5 while MIRA enriched DNA samples from normal tissue were labeled with Cy3. Keywords: Cancer Overall design: Methylation patterns of tumors; six patients with early stage breast cancer (DCIS)

从肿瘤DNA及配对正常对照中获取的甲基化基因组片段，采用甲基化-CpG岛恢复分析法（methylated-CpG island recovery assay, MIRA）进行富集；经扩增、标记后，在安捷伦（Agilent）CpG岛平铺寡核苷酸阵列上完成杂交。经MIRA富集的肿瘤样本以Cy5进行标记，而经MIRA富集的正常组织DNA样本则以Cy3标记。
关键词：癌症
实验设计概述：肿瘤的甲基化谱；纳入6名早期乳腺癌（导管原位癌，ductal carcinoma in situ, DCIS）患者