The chicken immature erythrocyte transcriptome. Gallus gallus

Transcriptional regulation is impacted by multiple layers of genome organization. Here, we identified and mapped out all the transcriptionally active chromosomal domains in the chicken immature erythrocyte genome, including the known β- and α-globin domains, by combining a powerful chromatin fractionation method with next generation DNA and RNA sequencing. Overall design: Two biological replicates of total RNA were sequenced to characterize genes in chicken erythrocyte cells

转录调控（transcriptional regulation）受多层基因组组织的影响。本研究结合高效染色质分级分离（chromatin fractionation）方法与新一代DNA及RNA测序技术，在鸡未成熟红细胞基因组中鉴定并定位了所有转录活跃的染色体结构域，其中包括已知的β-珠蛋白（β-globin）与α-珠蛋白（α-globin）结构域。实验整体设计：对总RNA进行两次生物学重复（biological replicates）测序，以表征鸡红细胞中的基因特征。