The impact of Prdm16 deletion on the transcriptome of the developing cochlear duct cells. The impact of Prdm16 deletion on the transcriptome of the developing cochlear duct cells

The PR domain containing 16 (PRDM16) is a key transcription regulator in the development of craniofacial, adipose, neural, and hematopoietic tissues. Our lab identified PRDM16 expression in the epithelial cells of the Kölliker’s organ (KO) starting around E13.5 and maintained until the disappearance of the KO around P10. Bulk RNA sequencing of cochlear duct cells at E14.5 followed by quantitative real time PCR and mRNA Fluorescence in-situ hybridization identified differentially expressed genes in Prdm16-null versus littermate control cochleae. Overall design: Cochlear duct mRNA profiles of embryonic day 14.5 (E14.5) wild type (WT), Prdm16-het and Prdm16-null mice

含PR结构域蛋白16（PRDM16）是颅面、脂肪、神经及造血组织发育过程中的关键转录调控因子。
本实验室于胚胎发育第13.5天（E13.5）左右在柯尔基器（Kölliker’s organ, KO）的上皮细胞中首次检测到PRDM16的表达，该表达持续至P10左右柯尔基器消失时终止。
本研究通过对胚胎第14.5天（E14.5）的耳蜗导管细胞开展批量RNA测序（bulk RNA sequencing），结合实时定量PCR（quantitative real time PCR）与mRNA荧光原位杂交（mRNA Fluorescence in-situ hybridization），鉴定出Prdm16纯合敲除（Prdm16-null）小鼠与其同窝对照小鼠耳蜗组织中的差异表达基因。
实验设计：针对胚胎发育第14.5天（E14.5）的野生型（WT）、Prdm16杂合敲除（Prdm16-het）及Prdm16纯合敲除（Prdm16-null）小鼠，对其耳蜗导管进行mRNA表达谱分析。