Ionizing radiation induced changes of miRNA expression in A549 lung cancer cells. Homo sapiens

We have employed microarray expression profiling as a discovery platform to identify microRNAs induced by radiation. Human A549 lung cancer cells were irradiated (2Gy/day for consistent 3days) and miRNA signature was identified that distinguished between control and radiation treated samples.Irradiated and un-irradiated A549 cells were harvested after 48 hr of treatment and microarray analysis was performed. Expression of five miRNAs (miR-30a, miR-30b, miR-30c, miR-30d, and miR-30d) from this signature was quantified in the same RNA samples by real-time PCR, confirming variability between control and radiation treated A549 cells. Overall design: Radiation induces miRNA expression in A549 cell line was measured at 48 hours after exposure to doses of 2Gy for 3days.

本研究以微阵列表达谱（microarray expression profiling）作为发现平台，筛选辐射诱导的微小RNA（microRNAs）。将人A549肺癌细胞予以辐照处理（每日2Gy，连续3天），鉴定得到可区分对照组与辐照处理组样本的miRNA表达特征谱。于处理48小时后收集辐照与未辐照的A549细胞，开展微阵列分析。通过实时定量PCR（real-time PCR）对该特征谱中的5种miRNA（miR-30a、miR-30b、miR-30c、miR-30d及miR-30d）进行定量验证，证实对照组与辐照处理的A549细胞间存在表达差异。整体实验设计：检测A549细胞系中辐射诱导的miRNA表达情况，辐照剂量为每日2Gy、连续3天，于暴露后48小时进行检测。