Tajima’s relative rate test.

The equality of evolutionary rate analysis between sequences A (OsMPK6) and B (ScMPK-A), with sequence C (CjMPK) were used for the analysis of fungal MAPKs with a few AtMAPKs and OsMAPKs as the representative of T-E-Y and T-D-Y motifs. Sequences A (DeMPK) and B (AtMPK1), with sequence C (CjMPK) were used for fungal MAPKs with all the representative AtMAPKs and OsMAPKs as per default selection on the MEGA program. In the case of all fungal MAPKs that contain the novel activation loop motif, the equality of the evolutionary rate was calculated between sequences A (687684) and B (91669), with sequence C (5240). A P-value less than 0.05 is often used to reject the null hypothesis of equal rates between lineages. The analysis involved 3 amino acid sequences. All positions containing gaps and missing datas were eliminated. Evolutionary analyses were conducted in MEGA5.

本研究针对序列A（OsMPK6）与序列B（ScMPK-A）开展进化速率均等性分析，以序列C（CjMPK）作为参照；本次分析选取少量AtMAPKs与OsMAPKs作为T-E-Y、T-D-Y基序的代表，用于真菌丝裂原活化蛋白激酶（Mitogen-Activated Protein Kinase, MAPK）的相关研究。针对序列A（DeMPK）与序列B（AtMPK1）、以序列C（CjMPK）为参照的分析，则结合分子进化遗传学分析软件（Molecular Evolutionary Genetics Analysis, MEGA）默认选择的全部代表性AtMAPKs与OsMAPKs，用于真菌MAPKs的相关研究。对于所有包含新型激活环基序的真菌MAPKs，以序列C（5240）为参照，计算序列A（687684）与序列B（91669）之间的进化速率均等性。通常以P值小于0.05作为拒绝谱系间进化速率均等性原假设的判定标准。本次分析共纳入3条氨基酸序列，所有包含比对间隙与缺失数据的位点均被移除。所有进化分析均通过MEGA5软件完成。