Nervous necrosis virus (NNV) detection results from infected grouper fish from 3 different fish farms.

aLocations of the grouper fish farms are shown in Figure S2 and the fish samples were collected in 2008 and 2009.b+, NNV-positive samples by microfluidic chip analysis; −, NNV-negative samples by microfluidic chip analysis.cThe first observation of viral nervous necrosis (VNN) clinical signs following sampling; +, groupers displayed VNN clinical signs; −, groupers did not display any clinical signs of disease; Clinical signs of VNN in larval-stage groupers were abnormal schooling and swimming behavior (whirling, spiraling) and loss of appetite.dThe tracking observation (disease outbreak) 2 weeks after the first observation from the same grouper fish farms; +, groupers displayed VNN clinical signs; −, groupers did not display any clinical signs of disease.

a. 石斑鱼养殖场的布设点位见图S2，鱼类样本采集于2008年与2009年。
b. "+"代表经微流控芯片检测呈神经坏死病毒（NNV）阳性的样本；"−"代表经微流控芯片检测呈神经坏死病毒（NNV）阴性的样本。
c. 采样后首次观测到的病毒性神经坏死症（Viral Nervous Necrosis, VNN）临床症状："+"表示石斑鱼出现VNN临床症状，"−"表示石斑鱼未表现出任何疾病临床症状；幼龄阶段石斑鱼的VNN临床症状包括集群异常、游泳行为异常（回旋、螺旋式游动）以及食欲减退。
d. 针对同一片石斑鱼养殖场首次观测后2周开展的追踪观测（疾病暴发情况）："+"表示石斑鱼出现VNN临床症状，"−"表示石斑鱼未表现出任何疾病临床症状。