Expression data from yeast treated with enediynes compared to gamma radiation

We are investigating the transcriptional response of yeast to treatment with enediynes or gamma radiation, which generate different extents of double or single strand breaks in DNA. We used microarrays to detail the global programme of gene expression underlying the DNA damage response in yeast Keywords: dose Yeast were grown to mid log phase and treated with enediynes or gamma radiation (in biological triplicate) resulting in similar extents of cell killing. The responses were compared to each other and we have deciphered a gene expression profile that is specific for double and single strand breaks in DNA.

我们正探究酵母经烯二炔(enediyne)或γ射线(gamma radiation)处理后的转录反应，这两种处理手段可在DNA中引发不同程度的双链断裂(double-strand breaks)与单链断裂(single-strand breaks)。本研究采用基因芯片(microarrays)详细解析酵母DNA损伤反应背后的全局基因表达调控程序。
关键词：剂量(dose)
将酵母培养至对数中期，分别用烯二炔(enediyne)或γ射线(gamma radiation)进行处理（设置3次生物学重复），两组处理的细胞杀伤程度相当。将两组转录反应进行相互比对，最终解析出特异性对应DNA双链断裂与单链断裂的基因表达谱。