Microbiome related to muscle strength

In this study, we developed a new method to overcome the limitations of traditional gut microbiome analysis, focusing on issues related to host genetic background and the use of the fecal microbiome as a proxy for the total gut microbiome. To minimize rapid changes in the microbiome due to various interacting factors, including genetic influences, in mice, the human fecal microbiota transplantation (FMT) method was applied to accurately assess strength-enhancing efficacy. We comparatively analyzed changes in the composition of the overall gastrointestinal (GI) microbiota of individual rats before and after FMT, and through subsequent proof-of-concept experiments conforming to Koch's postulates, we identified gut lineages associated with muscle strength improvement.

本研究开发了一种新方法，以克服传统肠道微生物组分析的局限性，重点关注与宿主遗传背景相关的问题，以及将粪便微生物组作为总肠道微生物组替代物的应用局限。为最小化小鼠体内因包括遗传影响在内的多种交互因素引发的微生物组快速变化，本研究采用人类粪便菌群移植（FMT）方法，以精准评估肌肉增强功效。我们对比分析了单只大鼠在FMT前后整体胃肠道（GI）微生物组的组成变化，并通过后续符合科赫法则的概念验证实验，成功鉴定出与肌肉力量提升相关的肠道谱系。