Acidosis-induced gene expression regulation in rat cells (in monoculture)

Acidic milieu induced gene expression regulation in proximal tube cells (NRKE) and kidney fibroblasts (NRKF) from rats. NRKE and NRKF were cultivated (in monoculture) with pH 7.4 (control) or pH (6.0) for 48h, before proceeding with RNAseq.

大鼠近端肾小管细胞（NRKE）与肾成纤维细胞（NRKF）中酸性环境诱导的基因表达调控。将NRKE与NRKF以单培养（monoculture）方式分别置于pH 7.4（对照组）及pH 6.0的培养环境中培养48小时，随后进行RNA测序（RNAseq）。