Effect of Cabergoline on Post-Lactational Involution and Long-Term Mammary Gland Behavior.

The aim of this study is to assess the impact of cabergoline on the intensity of short-term post-lactational involution and to explore long-term alterations in the epithelial dynamics of the glandular component in Brca1/P53-deficient mice. This study also delves into the transcriptomic changes associated with these processes. To further investigate the molecular changes occurring at these temporal points, RNA sequencing (RNA-seq) was conducted 1 day after post-lactation involution and again at 60 days. Multiparous Brca1/P53-deficient mice (Liu et al., 2007) with two pregnancies were administered intraperitoneal (IP) cabergoline at a dose of X on the same day of the second weaning. To study the short-term effect of cabergoline, organoids were generated 24 hours later, RNA was extracted, and RNA-seq (Novogene) was performed. Mice treated with cabergoline were compared with untreated controls. For the long-term effect study, the experiment was repeated in another group of mice. Organoids were generated 60 days post-weaning, including multiparous mice treated and untreated with cabergoline and age-equivalent nulliparous mice.

本研究旨在评估卡麦角林对泌乳后短期乳腺复旧强度的影响，并探究Brca1/P53缺陷小鼠腺体组分上皮动力学的长期变化。本研究同时深入剖析与上述过程相关的转录组学改变。为进一步探究这些时间节点下发生的分子变化，本研究分别于泌乳后复旧1天及60天开展了RNA测序（RNA-seq）。经两次妊娠的多产Brca1/P53缺陷小鼠（Liu等，2007）于第二次断奶当日，以X剂量经腹腔（intraperitoneal，IP）注射卡麦角林。为探究卡麦角林的短期效应，研究人员于24小时后构建类器官，提取RNA并开展RNA测序（RNA-seq，Novogene）。卡麦角林处理组小鼠与未处理对照组小鼠进行对照比较。针对长期效应的研究，本研究于另一组小鼠中重复实验。于断奶后60天构建类器官，样本涵盖经卡麦角林处理与未处理的多产小鼠，以及年龄匹配的未产小鼠。