Aberrant DNA Methylation and Overexpression of NR0B1 are Prognostic Biomarkers in KEAP1-Mutant Lung Adenocarcinomas

This dataset contains bisulfite Sanger sequencing results for a specific CpG-rich fragment of the NR0B1 gene promoter, analyzed in two human cell lines: A549 (lung adenocarcinoma) Calu-3 (lung epithelial) The purpose of this analysis was to evaluate the methylation status of individual CpG sites within this region. Included Files: .ab1 files: raw chromatogram outputs from direct bisulfite sequencing Excel spreadsheet summarizing methylation levels at each CpG site for both samples Reference sequence and protocol documentation Methylation Level Codes: 0 = Unmethylated 1 = <33% methylation 2 = 33–67% methylation 3 = >67% methylation 4 = ~100% methylation ? = Indeterminable Sequencing was performed using standard bisulfite PCR and BigDye 3.1 chemistry, with capillary electrophoresis carried out on an ABI 3730XL instrument. Data were analyzed using Polyphred and manually reviewed.本数据集包含针对NR0B1基因启动子特定CpG富集片段（CpG-rich fragment）的亚硫酸氢盐桑格测序（bisulfite Sanger sequencing）结果，相关分析在两种人类细胞系中开展： A549（肺腺癌细胞） Calu-3（肺上皮细胞） 本分析旨在评估该区域内单个CpG位点（CpG site）的甲基化状态。 数据集包含以下文件： .ab1格式文件：直接亚硫酸氢盐测序所得的原始色谱输出结果 Excel电子表格：汇总了两个样本各CpG位点的甲基化水平 参考序列及实验方案文档 甲基化水平编码规则如下： 0 = 未甲基化 1 = 甲基化比例<33% 2 = 甲基化比例33%~67% 3 = 甲基化比例>67% 4 = 甲基化比例约100% ? = 无法确定 测序采用标准亚硫酸氢盐PCR及BigDye 3.1化学试剂，通过ABI 3730XL测序仪完成毛细管电泳。数据经Polyphred软件分析，并经人工复核。