Potencial of different hydroxyapatites as biomaterials in the bone remodeling

Abstract Purpose: To analyze the therapeutic potentials of different hydroxyapatites used for the correction of bone defects in rats. Methods: Forty rats, male, albino wistar, were distributed in 4 groups. They were submitted to a 3.5 mm defect in tibia. They received low purity hydroxyapatite, Strontium hydroxyapatite and hydroxyapatite doped with gallium, having a seven day evaluation time. Histopathology slides were stained with hematoxylin-eosin, for morphological evaluation. Were analyzed inflammatory processes, necrosis, presence of osteoclasts and osteoblasts, presence of the material, presence of white cells, neovascularization and bone neoformation. Results: It was observed that the groups HAPSr and HAPGa, presented better results of trabecular bone, hyaline cartilage and bone marrow more organized. Conclusion: There was improvement in the repair of the bone defect produced, showing that these hydroxyapatites are effective osteoinductive, osteoconductive, osteintegrant agents and have biocompatibility, and may be indicated for use in defect repairs.

摘要
研究目的：分析不同羟基磷灰石（hydroxyapatite）用于修复大鼠骨缺损的治疗潜力。
研究方法：选取40只雄性白化Wistar大鼠，分为4组。于胫骨处构建3.5mm骨缺损模型，分别植入低纯度羟基磷灰石、锶掺杂羟基磷灰石（Strontium hydroxyapatite）及镓掺杂羟基磷灰石，评估周期为7天。制备苏木精-伊红（hematoxylin-eosin）染色的组织病理切片以开展形态学评价，分析指标包括炎症反应、坏死情况、破骨细胞与成骨细胞的存在状态、植入材料残留、白细胞浸润、新生血管形成及骨新生情况。
研究结果：观察显示，锶掺杂羟基磷灰石组（HAPSr）与镓掺杂羟基磷灰石组（HAPGa）的骨小梁、透明软骨及骨髓结构更具规整性。
研究结论：本研究证实受试骨缺损的修复效果得到显著提升，表明上述羟基磷灰石材料均为有效的骨诱导、骨传导及骨整合制剂，且具备良好生物相容性，可用于骨缺损修复临床应用。