Effect of miR-33 supression in hepatocytes in NAFLD

We perform RNAseq in NAFLD livers from WT and miR-33-AlbCRE conditional knock-out mice. Liver were isolated from mice after 3 months of feeding a choline deficient high fat diet to analyze the effect of miR-33 deficiency. Analyze changes in gene expression occuring in the liver in NAFLD and the regulation mediated by miR-33.

本研究对野生型（WT）与miR-33-AlbCRE条件性基因敲除小鼠的非酒精性脂肪性肝病（NAFLD）肝脏组织开展RNA测序（RNAseq）。实验中，受试小鼠经3个月胆碱缺乏高脂饲料饲喂后摘取肝脏组织，以此分析miR-33缺失的生物学效应，以及非酒精性脂肪性肝病肝脏内的基因表达变化与miR-33介导的基因表达调控机制。