RRBS MDACC Lung PreCancer

To study the evolution of DNA methylation at genome level and methylation intra-tumor heterogeneity (ITH) during early lung carcinogenesis, we performed multiregional reduced representation bisulfite sequencing (RRBS) of 62 resected lung nodules from 39 patients including atypical adenomatous hyperplasia (AAH, n=14), adenocarcinoma in situ (AIS, n=15), minimally invasive adenocarcinoma (MIA, n=22), and invasive adenocarcinoma (ADC, n=11).EGA study EGAS00001004610

为探究肺癌变早期阶段基因组水平DNA甲基化的演化规律与瘤内甲基化异质性（intra-tumor heterogeneity, ITH），我们对39例患者的62枚手术切除肺结节开展了多区域简化代表性亚硫酸氢盐测序（reduced representation bisulfite sequencing, RRBS），纳入样本涵盖不典型腺瘤样增生（atypical adenomatous hyperplasia, AAH，n=14）、原位腺癌（adenocarcinoma in situ, AIS，n=15）、微浸润性腺癌（minimally invasive adenocarcinoma, MIA，n=22）及浸润性腺癌（invasive adenocarcinoma, ADC，n=11）。本数据集隶属于欧洲基因组-表型档案库（European Genome-phenome Archive, EGA）下的研究项目EGAS00001004610。