Genes and mechanisms regulated by androgens as possible contributors to the male excess observed in autism [hNSCs]. Homo sapiens

We analyzed androgen receptor (AR) target genes on human neural stem cells (hNSCs) by ChIP-sequencing of AR IP after either DMSO (solvent), Testosterone 100nM, DHT 10 or 100nM 4 hours treatment in order to find the role of AR during brain development. Overall design: Examination of AR IP sequences after DMSO (1 technical replicate), Testosterone 100nM (AR agonist, 2 technical replicates), DHT 10nM (AR agonist, natural metabolite of testosterone, 2 technical replicates) or DHT 100nM treatment by deep sequencing using Illumina HiSeq 2500

本研究为探究雄激素受体（androgen receptor, AR）在脑发育过程中的功能，针对人类神经干细胞（human neural stem cells, hNSCs）中的AR靶基因展开分析。具体实验方法为：对经不同处理4小时的细胞样本进行AR免疫沉淀（AR IP）后，开展染色质免疫共沉淀测序（ChIP-sequencing）。处理分组包括二甲基亚砜（DMSO，溶剂对照）、100nM睾酮、10nM双氢睾酮（DHT）以及100nM双氢睾酮（DHT）。实验整体设计如下：采用Illumina HiSeq 2500平台进行深度测序，对各组的AR IP序列进行检测，其中二甲基亚砜对照组设置1次技术重复，100nM睾酮组（AR激动剂）、10nM双氢睾酮组（AR激动剂，睾酮的天然代谢产物）均设置2次技术重复，同时包含100nM双氢睾酮处理组。