Molecular phylogenetics of Maxillaria and related genera (Orchidaceae: Cymbidieae) based on combined molecular data sets

The orchid genus Maxillaria is one of the largest and most common of neotropical orchid genera, but its current generic boundaries and relationships have long been regarded as artificial. Phylogenetic relationships within subtribe Maxillariinae sensu Dressler (1993) with emphasis on Maxillaria s.l. were inferred using parsimony analyses of individual and combined DNA sequence data. We analyzed a combined matrix of nrITS DNA, the plastid matK gene and flanking trnK intron, and the plastid atpB-rbcL intergenic spacer for 619 individuals representing ca. 354 species. The plastid rpoC1 gene (ca. 2600 bp) was sequenced for 84 selected species and combined in a more limited analysis with the other data sets to provide greater resolution. In a well-resolved, supported consensus, most clades were present in more than one individual analysis. All the currently recognized minor genera of “core” Maxillariinae (Anthosiphon, Chrysocycnis, Cryptocentrum, Cyrtidiorchis, Mormolyca, Pityphyllum, and Tri...

兰花属Maxillaria是新热带地区最大且最常见的兰花属之一，但其当前的属界限和亲缘关系长期以来被认为是人为划分的。本研究通过对单独及合并的DNA序列数据进行简约分析，推断了Dressler（1993）定义的Maxillariinae亚族内的系统发育关系，重点关注广义的Maxillaria。我们分析了一个合并矩阵，包含核核糖体内部转录间隔区（nrITS）DNA、质体matK基因及侧翼trnK内含子，以及质体atpB-rbcL基因间隔区，涉及代表约354个物种的619个个体。我们对84个选定物种的质体rpoC1基因（约2600碱基对）进行了测序，并将其与其他数据集合并进行更有限的分析，以获得更高的分辨率。在分辨率高且支持度好的一致树中，大多数分支在多个单独分析中均有出现。