An extensive metagenomic dataset of sewage samples across five European cities: A resource for analyzing microbial communities, antimicrobial resistance and pathogens. European Longitudinal Sewage

Sewage metagenomics is a powerful tool for the proactive surveillance of potential pathogens and disease outbreaks, enabling rapid response strategies for threats like COVID-19. To unveil emerging patterns in sewage microbial communities, a comprehensive understanding of the microbial composition of sewage is imperative. Here, we present a substantial longitudinal dataset originating from five European cities, consisting of 278 sewage samples, undergoing short-read metagenomic sequencing. Additionally, metagenome-assembled genomes were reconstructed for each individual sample and sampling site, providing a selection of 2,332 unique, high- and medium-quality prokaryotic species, classified using the Genome Taxonomy Database. Furthermore, we conducted Hi-C sequencing on a subset of the samples.

污水宏基因组学（sewage metagenomics）是用于主动监测潜在病原体与疾病暴发的有力工具，可为新冠疫情（COVID-19）这类威胁制定快速响应策略提供支撑。为揭示污水微生物群落的新兴变化模式，全面掌握污水的微生物组成至关重要。本研究公开了一套源自欧洲五座城市的大型纵向数据集，包含278份污水样本，均经过短读长宏基因组测序（short-read metagenomic sequencing）。此外，我们针对每份单独样本及采样位点重建了宏基因组组装基因组（metagenome-assembled genomes），最终获得2332株独特的中、高质量原核生物基因组，并依据基因组分类数据库（Genome Taxonomy Database）完成分类鉴定。进一步地，我们还对部分样本开展了Hi-C测序。