qPCR

Research Background: This study aims to investigate the gene expression differences of microbial communities under different soil treatment conditions. The focus of the study is on the expression of specific functional genes (such as aioA, DrsB, arsM, arsC) under the addition of different materials.Experimental Design and Data Collection: We conducted qPCR experiments on 96 soil samples.qPCR Data Description: We performed absolute quantification of 4 target genes. Standard curve methods were used to determine gene expression levels. The data includes the Ct values and relative expression levels for each sample.Data Format and Content: The data has been uploaded as an Excel file, which contains the Ct values, gene names, reference gene Ct values, and relative expression levels for each sample. Each row represents one sample, and the columns include sample ID, Ct values for the target genes, and Ct values for the reference gene.Data Usage Restrictions and License: This dataset is shared under the CC BY-ND 4.0 license, allowing others to share the data as is but prohibiting modifications. If used, please cite the source as required.Additional Information: Please note that the accuracy of the data may be affected by experimental variations, particularly in sample handling and the selection of gene primers.

研究背景：本研究旨在探究不同土壤处理条件下微生物群落的基因表达差异，重点关注添加不同外源物质时特定功能基因（如aioA、DrsB、arsM、arsC）的表达情况。
实验设计与数据采集：我们对96份土壤样本开展了实时荧光定量聚合酶链反应（quantitative real-time PCR，qPCR）实验。
qPCR数据说明：我们对4个靶基因进行了绝对定量分析，采用标准曲线法确定基因表达水平，数据包含每份样本的循环阈值（cycle threshold，Ct）值与相对表达量。
数据格式与内容：本数据集已以Excel文件形式上传，内含每份样本的Ct值、基因名称、内参基因Ct值及相对表达量。数据每行对应一份样本，列信息包含样本编号、靶基因Ct值与内参基因Ct值。
数据使用限制与许可协议：本数据集采用CC BY-ND 4.0许可协议进行共享，允许他人原样分享数据，但禁止对数据进行修改。若使用本数据集，请按要求引用来源。
补充说明：请注意，实验操作偏差（尤其是样本处理与基因引物选择环节）可能会影响数据的准确性。