The role of the cAMP/PKA pathway and Com2 in regulating the activity of UASru.

Com2Δ cells did not grow on acetate medium. Therefore, com2 mutants and their isogenic wt strain were grown in SD medium to either logarithmic stage (SD) or stationary stage (SA). The results are expressed as relative β-galactosidase activity and represent the average data for three independent transformants. Strains used were as follows: wt (Y1265) and its isogenic strain gpr1Δ/gpr1Δ (Y1267), ras2Δ/ras2Δ (Y1270), cdc25Δ/cdc25Δ carrying CDC25ΔSH3 on a URA3 CEN vector (Y1338-2), a wt haploid (Y1890) and its isogenic strains com2Δ (Y1968) and com2S164AS88A (Y2052), a wt haploid (Y1214) strain and its isogenic strain sko1Δ (Y2063). These strains carry the lacZ reporter genes integrated within genomic LEU2. NT – Not Tested.

com2Δ缺失菌株无法在乙酸盐培养基上生长。因此，本研究将com2突变株及其同基因背景野生型（wild type）菌株接种于SD培养基中，分别培养至对数生长期（SD组）与稳定生长期（SA组）。实验结果以相对β-半乳糖苷酶活性表示，数据为三次独立转化子的平均值。所用菌株如下：野生型菌株Y1265及其同基因背景菌株gpr1Δ/gpr1Δ（Y1267）、ras2Δ/ras2Δ（Y1270）、携带URA3 CEN载体且载体上携带有CDC25ΔSH3片段的cdc25Δ/cdc25Δ菌株（Y1338-2）；野生型单倍体菌株Y1890及其同基因背景菌株com2Δ（Y1968）与com2S164AS88A（Y2052）；野生型单倍体菌株Y1214及其同基因背景菌株sko1Δ（Y2063）。上述菌株的基因组LEU2位点均整合有lacZ报告基因。NT——未检测。