Biological stress profiling of Japanese medaka (Oryzias latipes) using eRNA

We investigated the potential of environmental RNA (eRNA) to assess the health status of Japanese medaka fish (Oryzias latipes) exposed to linear alkenyl sulfate (LAS). Analyzing eRNA and organismal RNA (oRNA) in aquarium water for a short period (~12 h) after the onset of exposure. Conclusively, eRNA-seq using aquarium water emerges as a valuable non-invasive tool for analyzing physiological stress. The findings of this study lay the foundation for the further development of eRNA-seq technologies.

本研究探究了环境RNA（environmental RNA, eRNA）在评估暴露于直链烯基硫酸盐（linear alkenyl sulfate, LAS）的日本青鳉（Oryzias latipes）健康状态方面的应用潜力。实验于暴露起始后的约12小时短时长内，对水族箱水体中的环境RNA与生物体RNA（organismal RNA, oRNA）开展了分析。研究结果证实，基于水族箱水体的eRNA测序（eRNA-seq）是一种极具应用价值的非侵入性生理应激分析工具。本研究的发现为eRNA-seq技术的后续发展奠定了重要基础。