Next Generation Sequencing of Gene expression changes in U2OS osteosarcoma cells with PML silencing

We used next generation sequencing to analyze the gene expression changes in U2OS osteosarcoma cells expressing shRNA targeting the promyelocytic leukemia (PML) gene transcripts cDNA libraries of U2OS cells expressing control shRNA or shRNA targeting PML were generated from one biological replicate

本研究采用下一代测序技术，对表达靶向早幼粒细胞白血病（PML）基因转录本的短发卡RNA（shRNA）的U2OS骨肉瘤细胞的基因表达变化进行分析。本研究从一次生物学重复中，构建了分别表达对照短发卡RNA与靶向PML的短发卡RNA的U2OS细胞的互补DNA（cDNA）文库。