MicroRNA profiling of different TUT4 and TUT7 deficient mouse tissues/cells against controls. MicroRNA profiling of different TUT4 and TUT7 deficient mouse tissues/cells against controls

To study the impact of the proteins TUT4 and TUT7 on the miRNome, their genes were conditionally deleted in MEFs, ESCs, liver and bone marrow. Total RNA samples from the TUT4 and TUT7 deficient tissues/cells (together with their control tissues/cells) were used to generate microRNA libraries.

为探究TUT4、TUT7蛋白对微小RNA组（miRNome）的影响，研究人员在小鼠胚胎成纤维细胞（Mouse Embryo Fibroblasts, MEFs）、胚胎干细胞（Embryonic Stem Cells, ESCs）、肝脏及骨髓中对这两个基因实施了条件性敲除。随后采集TUT4、TUT7缺陷型组织/细胞及其对应的对照组织/细胞的总RNA样本，用于构建微小RNA文库。